Figure 2.

circNUP98 affects cell growth, migration, invasion and apoptosis of RCC cells. A, RCC cells were transfected as indicated, and the expression of circNUP98 was measured by RT‐PCR. B, Cell proliferation analysed by CCK‐8 assay after knockdown of circNUP98 by shRNA for indicated time in RCC cells. C, Colony formation assay was conducted after silencing of circNUP98 in RCC cells. D, Analysis of apoptosis by flow cytometry after silencing of circNUP98 in RCC cells for 48 h. E, Caspase‐3 activity was assayed after silencing of circNUP98 in RCC cells for 24 h. F, Western blot of caspase‐3 after down‐regulation of circNUP98 in RCC cells. G, Wound healing assay was performed after silencing of circNUP98 in RCC cells. H, Tranwell assay was performed after silencing of circNUP98 in RCC cells. I, The indicated proteins were assayed by western blotting after silencing of circNUP98. Data were presented as mean ± SD. Experiments were performed at least three times. *P < .05; **P < .01; ***P < .001