Figure 3.

circNUP98 functions a sponge of miR‐567. A, Subcellular fraction analysis of localization of circNUP96, GAPDH mRNA and U6 mRNA. B, The levels of different miRNAs were measured by RT‐PCR after silencing of circNUP98 in RCC cells. C, The expression of miR‐567 in human normal renal cells (293K) and RCC cell lines (A498, 769‐P, Caki‐2, Caki‐1 and 786‐O) were measured by RT‐PCR. D, The enrichment of circNUP98 and miR‐567 was measured by RIP assay in RCC cells. E, RCC cells were transfected miR‐567 or miR‐NC mimics for 24 h, and then, the levels of miR‐567 were measured by RT‐PCR. F, The predicted binding sites between miR‐567 and circNUP98 (left) and relative luciferase activity in RCC cells co‐transfected with wt or mut luciferase reporters and miR‐567 mimics or corresponding negative control. G, RCC cells were transfected with miR‐567 or miR‐NC inhibitors for 24 h, and then, the expression of miR‐567 was measured by RT‐PCR. H, RCC cells were transfected as indicated for 24 h, and the migration (left) and invasion (right) were measured by wound healing and Matrigel assay, respectively. I, RCC cells were transfected as indicated for 48 h, and cellular apoptosis was analysed. Data were presented as mean ± SD. Experiments were performed at least three times. *P < .05; **P < .01; ***P < .001