Figure 4.

PRDX3 is a target of miR‐567. A, RCC cells were transfected with miR‐NC or miR‐567 mimics for 24h, and mRNA levels of PRDX3, GPC5 and TBX4 were measured by RT‐PCR. B, RCC cells were transfected with sh‐NC or sh‐circNUP98 for 24 h, and the expression of circNUP98 was assayed by RT‐PCR. C, RCC cells were transfected with miR‐NC or miR‐567 mimics for 24 h, and the protein levels of PRDX3 were measured by Western blotting. D, The predicted binding sites between miR‐567 and PRDX3 3’UTR region. E, relative luciferase activity in RCC cells co‐transfected with wt or mut luciferase reporters and miR‐567 mimics or corresponding negative control. F, The enrichment of circNUP98, miR‐567 and PRDX3 mRNA was measured by RIP assay in RCC cells. G, RCC cells were transfected with sh‐NC or sh‐PRDX3 for 24 h, and the protein levels of PRDX3 were measured by Western blotting. H, After silencing of PRDX3, the proliferation of RCC cells was measured by CCK‐8 assay at indicated time‐point. I, After silencing of PRDX3 for 24 h, the migration and invasion of RCC cells were measured by wound healing and Matrigel assay, respectively. J, After silencing of PRDX3 for 24 h, the apoptosis of RCC cells was measured by flow cytometry. Data were presented as mean ± SD. Experiments were performed at least three times. *P < .05; **P < .01; ***P < .001