Figure 5.

Overexpression of PRDX3 reversed the effects of silencing of circNUP98. A, RCC cells were transfected with pcDNA3.1 or pcDNA.PRDX3 for 24 h, and the protein levels of PRDX3 were measured by Western blotting. B, RCC cells were transfected as indicated, and the proliferation of cells was measured by CCK‐8 assay at indicated time‐points. C, RCC cells were transfected as indicated, and migration and invasion of cells were measured by wound healing assay and Matrigel assay, respectively. D, RCC cells were transfected as indicated, and cellular apoptosis was measured by flow cytometry. E, RCC cells were transfected as indicated, and cellular lysates were subjected to Western blotting with indicated antibodies. Data were presented as mean ± SD. Experiments were performed at least three times. *P < .05; **P < .01; ***P < .001