Figure 6.

STAT3 induces the expression of circNUP98 in RCC cells. A, RCC cells were transfected with pcDNA3.1 or pcDNA.STAT3 for 24 h, and the mRNA levels of STAT3 were measured by RT‐PCR. B, RCC cells were transfected with pcDNA3.1 or pcDNA.STAT3 for 24 h, and the protein levels of STAT3 were measured by Western blotting. C, RCC cells were transfected with pcDNA3.1 or pcDNA.STAT3 for 24 h, and the expression of circNUP98 was measured by RT‐PCR. D, RCC cells were transfected with sh‐NC or sh‐STAT3 for 24 h, and the mRNA levels of STAT3 were measured by RT‐PCR. E, RCC cells were transfected with p sh‐NC or sh‐STAT3 for 24 h, and the protein levels of STAT3 were measured by Western blotting. F, RCC cells were transfected with sh‐NC or sh‐STAT3 for 24 h, and the expression of circNUP98 was measured by RT‐PCR. G, The predictive promoter region of NUP98, top; the reporter constructs of wild‐type Luc and its mutated derivatives are also shown in bottom. H, The interaction between STAT3 and NUP98 promoter was validated through luciferase reporter assay. I, The binding ability between STAT3 and NUP98 promoter was testified by ChIP assay in RCC cells. Data were presented as mean ± SD. Experiments were performed at least three times. **P < .01; ***P < .001