FIGURE 4.

Breast cancer cells (MCF‐7)‐derived exosomes‐encapsulated miR‐27a‐3p elevated PD‐L1 expression in macrophages in vivo. A, Flow cytometry was used to evaluate macrophages. B, Confocal microscopy was used to observe exosomes uptake by macrophages (×400). C, Flow cytometry was used to determine exosomes uptake by macrophages; D, miR‐27a‐3p and PD‐L1 expression in co‐cultures of exosomes and macrophages determined using RT‐qPCR, *P < 0.05, vs Exo‐mimic NC. #P < 0.05 vs Exo‐inhibitor NC. E, PD‐L1 protein expression in macrophages co‐cultured with exosomes was determined using Western blot assay. *P < 0.05 vs Exo‐mimic NC. #P < 0.05 vs Exo‐inhibitor NC. Measurement data were presented as mean ± standard deviation, and ANOVA was utilized to compare data among multiple groups, followed by Tukey's post hoc test. Experiments were conducted in triplicates