Figure 1.
Anticancer Effect of Valeric Acid (VA) in Cell Proliferation and Colony Formation Assays
(A) The IC50 values were calculated based on dose-response data (cell absorbance) at 72 h. Liver cancer cell lines Hep3B, SNU-449, and HepG2 were sensitive to the treatment of VA. (B) Images from the colony formation assay using Hep3B, SNU-449, and HepG2 cells. (C) The relative colony formation efficiency showed a significant reduction (∼70%) of colonies formed after VA treatment in all 3 liver cancer cell lines tested. Data are presented as the mean ± standard deviation (SD); NS, not significant; NC, negative control.