Figure 3.

VA Restrained the 3D Formation Ability of Liver Cancer Cells

(A and B) The representative 3D spheroid models of Hep3B (A) and SNU-449 (B) cells treated by VA and NC. The relative 3D formation efficiency calculated from both the cross-section area and fluorescence values showed a significant reduction at all time points (p < 0.01) in VA-treated groups compared to control groups. (C and D) Relative inhibition rates of SNU-449 and Hep3B in response to VA were calculated by comparing the cross-section area (C) and fluorescence values (D) of VA groups to NC at 24, 48, 72, and 96 h, respectively. All assays were performed in triplicate.