FIGURE 7.

Klotho overexpression protects from electrical cardiac and Ca²⁺ cycling alterations induced by chronic kidney disease (CKD). (A–C) Mean serum levels of (A) blood urea nitrogen (BUN), (B) phosphates (Pi), and (C) fibroblast growth factor‐23 (FGF‐23) in Sham‐Tg‐Kl (N = 5 mice) and Nfx‐Tg‐Kl (N = 7 mice). (D) Mean values of Ca²⁺ spark frequency in Sham‐Tg‐Kl (995 sparks, n = 54 cells/N = 5 mice) and Nfx‐Tg‐Kl (893 sparks, n = 55 cells/N = 6 mice). (E) Occurrence of SCR in Sham‐Tg‐Kl (n = 54 cells/N = 5 mice) and Nfx‐Tg‐Kl (n = 55 cells/N = 6 mice). (F) Mean values of caffeine‐evoked Ca²⁺ transients amplitude expressed as peak (F/F ₀) in Sham‐Tg‐Kl (n = 40 cells/N = 5 mice) and Nfx‐Tg‐Kl (n = 34 cells/N = 6 mice). (G) Mean values of peak (F/F ₀) of electrically evoked Ca²⁺ transients in Sham‐Tg‐Kl (n = 64 cells/N = 5 mice) and Nfx‐Tg‐Kl (n = 55 cells/N = 6 mice). (H) Percentage of cell contraction of Sham‐Tg‐Kl (n = 56 cells/N = 5 mice) and Nfx‐Tg‐Kl (n = 52 cells/N = 6 mice). Data are shown as mean ± SEM. ^* P < 0.05, significantly different from Sham‐Tg‐Kl