Fig. 1. Effects of Cur, Hes, and their combination on cell viability and proliferation. MTT assays were performed to evaluate cell viability and proliferation. (A) SY5Y cells were treated with Dg (300 mM or 400 mM) for 48 h. (B, C) SY5Y cells were pretreated with Cur or Hes (0.3, 1, or 3 μM) for 24 h, and then were treated with 300 mM Dg for 72 h. Data are represented as the mean ± SEM. Letters label the bars which represent significant differences (P < 0.05).

Ctrl, control; Dg, D-galactose; Cur, curcumin; Hes, hesperetin; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide; SEM, standard error of the mean.