Fig. 2. Effect of Cur (1 μM) and Hes (1 μM) on synaptic loss and neuronal degeneration in Dg-induced aged SH-SY5Y cells. (A) Representative images (100× magnification) are shown. Neurite length was measured by Image J software. (a) Ctrl; (b) D-gal; (c) Cur; (d) Hes; (e) Combi; (f) Quantified average neurite length from each group. (B, C) The protein expressions of neuronal markers, β-tubulin III, p-ERK, and NEFH were analyzed by Western blot assay. α-tubulin was used as a loading control. (B) Representative blots, (C) quantified proteins levels. Data are represented as the mean ± SEM. Letters label the bars which represent significant differences (P < 0.05).
Ctrl, control; Dg, D-galactose; Cur, curcumin; Hes, hesperetin; Combi, curcumin + hesperetin; ERK, extracellular signal-regulated kinases; NEFH, neurofilament heavy polypeptide; SEM, standard error of the mean.