Figure 6.

Myoepithelial cells cultured from thrombospondin 1 null (TSP1^−/−, alias Thbs1^−/−) mice have altered Ca²⁺ stores compared with wild-type (WT) mice. Myoepithelial cells cultured from C57BL/6J (WT) or TSP1^−/− mice were stimulated with thapsigargin in the absence of extracellular Ca²⁺ and intracellular [Ca²⁺] was measured. Change in peak intracellular [Ca²⁺] (A) and the area under the curve (B) are shown. Extracellular Ca²⁺ was reintroduced and the rate of refilling of the stores measured (C). Data are expressed as means ± SD. n = 3 independent experiments performed in duplicate. ∗P < 0.05 versus WT. KCl, potassium chloride.