Figure 2.

ATM activity regulates DDX5 at DSBs. Cells harboring the mCherry-LacI-FokI reporter system were treated with the inhibitors of the three PIKK family of kinases (ATM, ATR or DNA-PK) (A, B) or transfected with indicated siRNAs (C). DDX5 exclusion from the FokI-induced DSBs was analyzed as described in the ‘Materials and Methods’ section. A typical image is shown for each sample. The fluorescence intensity of DDX5 at DNA damage sites relative to an unirradiated area was quantified. Data show the mean relative fluorescence intensity ± SE of ∼120 cells per condition from three independent experiments. Statistical significance was assessed using Student’s t-test: *P < 0.05; ***P < 0.001; n.s., not significant.