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. 2020 Sep 28;52(9):1627–1636. doi: 10.1038/s12276-020-00507-5

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© The Author(s) 2020

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Fig. 2 — a Chromatogram of protein elution. Protein adsorbed on a His-Trap column was eluted by adding imidazole. The eluted protein was collected in five fractions (E-1 to E-5) based on A₂₈₀. b The presence of rmIL-1Ra in each fraction was analyzed by Western blotting using an anti-His-tag antibody. c Results of analysis of E-3 by SDS-PAGE (CBB staining). Secretory rmIL-1Ra (black arrowhead: 21.8 kDa).