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. 2020 Sep 28;52(9):1627–1636. doi: 10.1038/s12276-020-00507-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — EL4.NOB-1 cells (2 × 10⁵) were stimulated with IL-1β (200 pg/well) and various concentrations (37.25–250 ng/well) of purified rmIL-1Ra, commercial mIl-1Ra, or commercial hIL-1Ra. Thereafter, the mIL-2 concentration in the culture supernatant was determined by ELISA. Data are representative of two independent experiments and presented as the mean ± SD. Means denoted by different letters differed significantly (p < 0.05). n.d. not detected. Mouse (gmLAB), rmIL-1Ra purified from NZ-IL1Ra; mouse (commercial), commercially available mIl-1Ra; human (commercial), commercially available hIL-1Ra.