Figure 7.

HDAC6 Acts Downstream of Smad7 to Promote c-Jun Expression and Invasion

(A and B) Smad7^−/− cells transfected with pcDNA3 or HDAC6 plasmids and treated or not with TGF-β were lysed, and cell lysates were subjected to RT-PCR by using c-Jun primers (A) or IB for c-Jun, HDAC6, and α-tubulin (B).

(C) Smad7^−/− cells transfected with EGFP and HDAC6 or pcDNA3 plasmids were stimulated or not with TGF-β and subjected to an invasion assay, as described in Methods. Scale bar, 100 μM.

Bar graph are means ± SEM from three independent experiments. One-way ANOVA was used as the statistical test. ∗p < 0.05, ∗∗∗p < 0.001.