FIG. 1.

Long LPS stimulation in MSCs increases the expression of inducible nitric oxide synthase (iNOS). a MSCs were stimulated with LPS (500 ng/ml) for 1, 24, and 48 hours (h), then washed with PBS and cultured again in complete α-MEM medium for next 12, 24, and 48 h. b RNA was obtained, and quantitative PCR (qPCR) assays for iNOS and c IL6 genes were performed. Data are expressed as mean ± SEM; n = 3, N = 3 biological replicates; ***p < 0.001, ****p < 0.0001 (inside each experimental group). δδδp < 0.001 (between each experimental group), derived by one-way ANOVA, Kruskal-Wallis ad hoc post test