FIGURE 3.

EPS analysis of Streptococcus mutans. (a‐b) Three‐dimensional reconstructions of live bacteria and EPS in biofilms of standard S. mutans and one representative clinical strain at 4 h, 6 h, 12 h, and 24 h. EPS is labeled in red (Alexa Fluor 647), and bacterial cells are labeled in green (SYTO9). (c‐d) EPS biomasses of standard S. mutans and 10 S. mutans clinical strains at 4 h, 6 h, 12 h, and 24 h. EPS biomasses were calculated according to 5 random sites in each CLSM micrograph image. Each determination was repeated three times