TABLE 1.
Primers for candidate sRNAs and 16S rRNA
| sRNA ID | Primers | |
|---|---|---|
| Forward | Reverse | |
| sRNA0698 (Zhu et al., 2018) | CTATTTCTGTTCTATTTTACCACA | Universal primer |
| sRNA0593 (Zhu et al., 2018) | CGCCAATCATTTCATTTTCCA | CCTACGTTTCCCGTGCCTAA |
| sRNA0074* | TACTGGAATAATGTTTAATTTTACT | Universal primer |
| sRNA0522* | CAATAGTAATAAGGTAAAGTGCG | GTATCTCGTAAATACTACAAAGAATT |
| sRNA0426* | ATTGGATAAGACCGTTACACA | AAATAGCGAGACAAGAAAGTT |
| sRNA0413* | AATAATAAGTCCGCAAAAATC | AAGGTGGATTAGGTAAAGATG |
| sRNA0650* | TTAGCATCTTTTACATCACAATA | TGATTCTTCTTTATGGGACA |
| sRNA0146* | AGCTAGTTGCTATAATTAATAATTT | TTCTCTTCAGTTAGACAATCTCT |
| sRNA0215* | TTGTGAAGCTCTCAATAAGTT | GATGTATCCAATGAATCAGTGA |
| sRNA0120* | TAAGCGTAAGCGGCAAAACT | AATAGCTGGGCTTCAGGTGC |
| sRNA0118* | AATATTGATTTTGACCTGCAT | GATTTTAGGCTAACTTTTGAGAT |
| sRNA0379* | AGTGCTTCTTCAATTTTATCCATC | GGCAAGGATAGAATGGTTGT |
| sRNA0250* | GCCATTTAAGATTCGGACTA | AGGAAGTGAATAAGTATGAAAGT |
| sRNA0301* | CTAAAGGGCAATAAAATATGTGA | GAAGCGTTTCCTATAAATTCTAT |
| sRNA0600* | TGTATTTGTTTCGGACCTTA | CGCTATTACGCGATATTCT |
| sRNA0656 (Zhu et al., 2018) | TATGGGGGATAAGATATGCTATGAT | Universal primer |
| sRNA0330 (Zhu et al., 2018) | TTTATTAGAAAGGAACAGTTTTG | Universal primer |
| sRNA0187 (Zhu et al., 2018) | CGTTCCGTCAAATAACCAAAGTG | AAGGAGAATGGTAATTCCGCTTT |
| sRNA0329 (Zhu et al., 2018) | GCAAAACTGTTCCTTTCTAATAA | Universal primer |
| sRNA0679 (Zhu et al., 2018) | AATCTCAAGCAAAGACTTTTTAGA | Universal primer |
| 16S rRNA | CTTACCAGGTCTTGACATCCCG | ACCCAACATCTCACGACACGAG |
*
The primers were designed by the technical staff from the TakaRa company. The universal primer was commercially supplied with the Mir‐X miRNA qRT‐PCR SYBR kits (TaKaRa).