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The apoptosis rate was measured using annexin V-FITC after liraglutide (Lir) treatment and hypoxia/reoxygenation (H/R) in H9c2 cells with Notch1 depletion (a). Apoptosis rate = annexin V-positive cells/PI-negative (living) cells (b). The GLP-1R and Notch1 protein levels were determined by western blotting (c and d). n = 3, *P < 0.05, **P < 0.001.
