FIGURE 4.

Inflammation, lipid and fibroblast‐related gene regulation by acute ACh treatment in human subcutaneous and epicardial adipogenesis‐induced stromal vascular cells (SVC). Interleukin 6 (IL‐6) and monocyte chemoattractant protein 1 (MCP1) (A, D); perilipin 1 (PLINA), peroxisome proliferator‐activated receptor gamma (PPARγ), fatty binding protein 4 (FABP4) and CCAAT/enhancer‐binding protein beta (C/EBPβ) (B, E); and collagen type I alpha II (COL1A2) and pre‐adipocyte factor 1 (PREF1) (C, F) mRNA expression levels relative to actin (ACTB) in epicardial SVC (A, B, C) or subcutaneous SVC (D, E, F) (n = 12). Dot plots with bar depict individual values and mean ± SEM. Differences between vehicle and treatment were evaluated by paired t test and represented with a line and asterisks. Significant values are expressed as *P < .05