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. 2020 Aug 7;24(18):10958–10969. doi: 10.1111/jcmm.15727

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© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Neutrophil migration by acute ACh treatment and lipid accumulation by chronic ACh treatment. Migrated cells count relative to basal control were analysed after acetylcholine (ACh) or conditioned medium from ACh treated or not ‐subcutaneous and epicardial stromal vascular. Complement component 5 alpha (C5a) was used as positive control (A). Representative images showing AdipoRed lipid staining in SVC from subcutaneous (SAT) and epicardial (EAT) adipose tissue after ACh treatment for 21 days. AdipoRed Assay was quantified by fluorimetry from 5 independent patients (n = 5). Dot plots depict individual values and mean ± SEM. Comparisons between control and treatment were analysed by paired t tests. Significant values represent *P < .05 and **P < .01 (B)