(A) vGATL10a reporter mice were used to identify GABA neurons in layer 5 of the IC for whole-cell recording. (B) In GABA neurons, 10 min bath application of dynorphin A (Dyn, 300 nM) did not alter the frequency or (C) amplitude of spontaneous excitatory postsynaptic current (sEPSC) from baseline (BL). (D) Representative traces of sEPSCs and (E) sIPSCs at baseline (black) and following Dyn (blue) wash-on. (F) There was no effect of Dyn on spontaneous inhibitory postsynaptic current (sIPSC) frequency, (G) amplitude, or (H) sEPSC/sIPSC (E/I) ratio in layer 5 IC GABA neurons. Females, open circles; males closed circles; n = 2–3 mice/sex; BL, baseline; Dyn, dynorphin A