Table 5.

The effect of different concentrations of NSE on embryonic development in the control and PCOS mice oocytes during in vitro maturation

Groups	Fertilization (%)		2-cell (%)		8-cell (%)		Blastocyst (%)
	Control	PCOS	Control	PCOS	Control	PCOS	Control	PCOS
Control (0)	78.50 $\pm$ 2.45	68.50 $\pm$ 2.34	66.83 $\pm$ 2.84	55.16 $\pm$ 3.57	49.00 $\pm$ 4.11	40.67 $\pm$ 4.12	42.50 $\pm$ 3.16	30.83 $\pm$ 2.54
NES 1 μg/ml	87.50 $\pm$ 2.67 (p = 0.112)	75.16 $\pm$ 2.18 (p = 0.225)	73.17 $\pm$ 3.52 (p = 0.276)	69.83 $\pm$ 2.77 (p = 0.167)	51.66 $\pm$ 3.47 (p = 0.368)	45.00 $\pm$ 4.10 (p = 0.651)	46.33 $\pm$ 2.86 (p = 0.425)	37.00 $\pm$ 3.52 (p = 0.327)
NES 50 μg/ml	88.83 $\pm$ 1.85 (p = 0.145)	82.17 $\pm$ 1.9 (p = 0.027)	81.50 $\pm$ 2.86 (p = 0.025)	77.50 $\pm$ 2.41 (p = 0.015)	65.00 $\pm$ 3.01 (p = 0.033)	63.33 $\pm$ 3.86 (p = 0.017)	57.83 $\pm$ 2.70 (p = 0.037)	50.33 $\pm$ 2.94 (p = 0.008)
NES 100 μg/ml	76.17 $\pm$ 3.10 (p = 0.625)	71.17 $\pm$ 2.98 (p = 0.341)	69.00 $\pm$ 3.52 (p = 0.725)	59.50 $\pm$ 3.22 (p = 0.635)	48.00 $\pm$ 3.61 (p = 0.976)	44.67 $\pm$ 3.45 (p = 0.725)	42.50 $\pm$ 3.56 (p = 0.951)	37.50 $\pm$ 3.44 (p = 0.542)
Percentage rates of fertilization, 2-cell, 8-cell, and blastocyst formation of control and PCOS mice oocytes cultured in maturation medium, supplemented with 0, 1, 50, and 100 μg/ml NSE. NES: Nigella sativa extract; PCOS: Polycystic ovary syndrome. Data are presented as Mean $\pm$ SEM. P: P-value between each concentration group of NSE and control (0 μg/ml of NSE) by one-way ANOVA