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. 2020 Sep 10;8:564731. doi: 10.3389/fbioe.2020.564731

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Copyright © 2020 Wang, Wang, Zhou, Sun, Zhu, Duan, Chen, Guo, Zhang and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Masson’s trichrome and BMP-2 staining of bone defect sections and quantitative analysis of bone deposition 30, 60 and 90 days post-implantation of CHA/SF/GCS/DF-PEG hydrogel or CHA/SF/GCS/DF-PEG hydrogel associated with hucMSC-derived exosomes. Representative images of Masson’s trichrome staining (A) and BMP-2 staining (B) of the bone defect sections. In Masson’s trichrome staining, the new bone tissue is observed in red and the collagen tissue in blue-green. Quantitative analysis of the percentage of new bone area formed (C) and BMP-2 stained area (D) in the control and in the two experimental groups in the three observed times. a, P < 0.05 compared to the control group; b, P < 0.05 compared to the hydrogel group.