Figure 4.

SP1/miR‐326 modulated viability and proliferation of OS cells. MG63 and 143B cells were transfected with siRNA‐SP1, siRNA‐SP1 together with miR‐326 inhibitors, miR‐326 mimics, miR‐326 inhibitors and negative control. A, PCR was performed to measure miR‐326 expression level. Cell viability and proliferation were evaluated using MTT (B) and Edu assays (C), respectively. Statistical analysis was performed at the time point of 72 h in MTT assay and at 48 h in Edu assay. *P < .05, **P < .01 vs WT group that did not undergo transfection