Figure 5.

Suppressed miR‐130a promotes neuron viability and decelerates apoptosis of in a cell mode of OGD, and reduced XIAP abolished these effects. A, Primary neurons (× 200); B, Neurons were identified by immunohistochemical staining (× 200); C, miR‐130a and XIAP expression in neurons was determined using RT‐qPCR; D, XIAP protein expression in neurons was assessed by Western blot analysis; E, The neuron viability was measured by MTT assay; F, Flow cytometry was used to assess the apoptosis rate of neurons. a, P < 0.05 vs the control group; b, P < 0.05 vs the OGD group; c, P < 0.05 vs the OGD + miR‐130a inhibitors group. The results were expressed as mean ± standard deviation and comparisons among groups by one‐way ANOVA, followed by Tukey's post hoc test