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. 2020 May 27;34(9):e23378. doi: 10.1002/jcla.23378

Figure 2.

Figure 2

Reciprocal regulation between GACAT3 and LINC00152 through miR‐103. A, GACAT3 and LINC00152 share common binding sites of three miRNAs. Common miRNA binding sites were predicted by microT and miRcode. B, GACAT3 and LINC00152 regulation by the miRNAs. The miRNA mimics of miR‐103, miR‐128, and miR‐138 were applied to HCT116, and then GACAT3 and LINC00152 expression was determined by qRT‐PCR. C, Direct regulation of GACAT3 and LINC00152 by miR‐103 shown by a dual luciferase reporter assay. Mimics of miR‐103 were applied after the transfection of plasmid pGL3‐GACAT3 or pGL3‐LINC00152 into 293T cells, and luciferase assay was performed. D, GACAT3 and LINC00152 have the reciprocal regulatory effects. Knockdown was performed by specific siRNAs of GACAT3 and LINC00152, and then their expression was determined by qRT‐PCR. Data represent the mean ± SD of three independent experiments. *P < .05; **P < .01