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. 2020 Aug 6;24(18):10756–10767. doi: 10.1111/jcmm.15700

Figure 5.

Figure 5

The up‐regulation STAT pathway by rIL‐27 resulted in anti‐apoptosis of alloreactive spleen cells. Alloreactive MLR were performed with treated by rIL‐27, ruxolitinib, SH‐4‐54 and anti‐IL‐27 p28 Ab for 72 h. IL‐27 group treated with rIL‐27. IL‐27 + R group treated with rIL‐27 and ruxolitinib. IL‐27 + S group treated with rIL‐27 along with SH‐4‐54. IL‐27 + Ab group treated with rIL‐27 and anti‐IL‐27 p28. A, STAT1, STAT3, STAT5, Bcl‐xL and Pim2 expression were detected by Western blot. B, Band intensities in Western blot for STAT1, STAT3, STAT5 expression were measured ImageJ. C, The band intensities in Western blot for Bcl‐xL and Pim2 expression were measured ImageJ. D, Alloreactive spleen cell apoptosis detected by flow cytometry. Fold change means the ratio of apoptotic cells in each group to those in IL‐27 group. *P < .05, **P < .01, ***P < .001 vs Control group. # P < .05, ## P < .01, ### P < .001 vs IL‐27 group