Figure 5.

Curcumin up‐regulated the expression of miR‐143 and miR‐124, which subsequently inhibited NF‐kB and ROCK1 expression, respectively. A‐B, Bisulphite sequencing showed reduced DNA methylation of miR‐143(A) and miR‐124(B) promoters in primary chondrocytes treated with a higher concentration of curcumin. C‐D, Luciferase assay showed an elevated transcription efficiency of miR‐143(C) and miR‐124(D) promoters in primary chondrocytes treated with a higher concentration of curcumin. E, Potential target site of miR‐143 on NF‐kB 3’ UTR. F, The luciferase activity of WT NF‐kB vector was inhibited by miR‐143. G, Potential target site of miR‐124 on ROCK1 3’ UTR. H, The luciferase activity of WT ROCK1 vector was inhibited by miR‐124