FIGURE 4.

P2X7R overexpression in colorectal cancer (CRC) cells increases CSC characteristics. A, Western blotting of the indicated proteins in CT26‐Con and CT26‐mP2X7R cells. B, mRNA levels of the indicated genes in CT26‐Con and CT26‐mP2X7R cells were analysed by real‐time PCR. C, Analysis by flow cytometry of CD44 and CD166 expression in CT26‐Con or CT26‐mP2X7R cells. D, Tumorsphere formation ability of CT26‐Con or CT26‐mP2X7R cells. E, Tumorsphere formation ability of HCT116‐Con and HCT116‐P2X7R cells. F, Tumorsphere formation ability of HCT116‐sh Con and HCT116‐sh P2X7R cells. The results are expressed as the means ± SD of 3 independent experiments. **P < 0.01