FIGURE 1.

Behaviour of MSC after subcutaneous transplantation in the interscapular region. A, Fluorescent signal of the labelled MSC graft 30 min after the transplantation, as assessed by in vivo imaging. B, representative ex vivo fluorescence imaging of mouse organs at 7d post‐transplantation of CMTPX‐labelled MSC (right) or with vehicle, as control (left); BAT, brown adipose tissue; WAT, white adipose tissue. C, total radiant efficiency values of spectral‐unmixed signal in the MSC aggregate, WAT and lymph node (LN) at 7d after transplantation. D, survival curve for two cell doses (low and high) of grafted cells determined by the in vivo bioluminescent (BLI) signal of Luc‐expressing MSC measured as average radiance. E, representative images of in situ vascularization of MSC aggregates at 7d post‐transplant (after skin excision and graft exposure). F, in vivo assessment of graft‐induced inflammation by quantification of myeloperoxidase‐based bioluminescent signal after i.p. injection of lucigenin in mice receiving low and high MSC doses (**P < .01, Student's t test)