FIGURE 1.

Screening of natural compounds against STZ‐induced cytotoxicity. A, Dose‐response curve of N2a cell viability upon treatment with different concentrations of STZ. The lethal concentration 50 (LC₅₀) of STZ for the N2a cells used in this study is shown. B, Overview of primary screening data. N2a cells were treated with LC₅₀ of STZ alone (no treatment) or in combination with individual natural compounds at two concentrations (1 and 5 μmol/L). Cell viability was analysed by MTT assay and normalized to DMSO‐treated control cells. Data represent mean ± SD of three biological replicates. **P < 0.01; *P < 0.05. C, Dose‐response curve of N2a cell viability upon treatment with different concentrations of STZ alone or in the present of 5 μmol/L ISO. The lethal concentration 50 (LC₅₀) of STZ under the two conditions is shown