Figure 5.

Ad‐CAIX^promotor‐AIM2 inhibited the tumour growth of OSRC‐2 cell‐xenografted nude mice. The OSRC‐2‐xenografted nude mice were injected intratumourally Ad‐CAIX^promotor‐AIM2 or control with or without inhibitor on tumour reached about 50 mm³ in volume. (A) Caspase‐1 activity was detected in tumour tissues. (B) The levels of IL‐1β were examined in tumour tissues. (C) Tumour progression of OSRC‐2‐xenograft was evaluated by measurement of tumour volume twice a week from day 0 to day 35. (D) Tumour volumes were measured at day 35 after tumour inoculation. (E) The tumour weights were monitored at day 35 after tumour inoculation. (F) Tumour inhibition rate. (G) At day 2 or 7 after the initial administration, the serum were collected, the levels of IL‐6 were determined by ELISA assay. (H) At day 35 after tumour inoculation, the pathology was evaluated by H&E staining of heart, kidney or liver tissues. Each experiment was performed independently at least three times and the results of one representative experiment are shown. Data are means ± SD, *P < .05, **P < .01 and ***P < .001; ns, no significant difference