FIGURE 1.

Time of simvastatin (SV dosing influences liver pathological characteristics and the expressions of lipid metabolic and drug transport genes. SV was administered to high‐fat diet (HFD) + SV groups at ZT1 or ZT13, respectively, for nine weeks, and at the same time, 0.5% CMC was administrated to normal diet (ND) and HFD groups (n = 5). A, Flow diagram of animal experiments. B, Liver H&E and ORO staining (200×). C, The mRNA expression levels of cholesterol and lipid metabolic genes. D, The expression of Hmgcr at transcriptional and translational levels. E, The expression of Mdr1 at transcriptional and translational levels. F, The expression of Slco2b1 at transcriptional and translational levels. All values are expressed as means ± SD. *represents the comparison of ND vs HFD (*P < .05, **P < .01); ^#represents the comparison of HFD vs HFD + SV (^# P < .05, ^## P < .01)