Figure 2.

High Expression of lncRNA‐HEIH Promotes ESCC Cell Proliferation In Vitro and Vivo. Eca109 cells and TE13 cells stably transfected with lentivirus encoding lncRNA‐HEIH (A) and whose transfected with siRNA against lncRNA‐HEIH (B) were seeded in 96‐well plates, and cell proliferation was assessed daily for three days using the CCK‐8 assay. Changes in the proliferation marker PCNA were shown by western blotting analysis and normalized to β‐actin (C) and (D). The in vivo models used were xenograft transplanted nude mouse tumour models of human ESCC growth established with lncRNA‐HEIH up‐regulated Eca109 cells (Over‐1, overexpression‐1). (E) Photographs of tumours by imaging with the IVIS system. A representative luciferase signal was captured in each group at 3 weeks after the cell injection. A photograph of the tumours is also presented (F). (G) Effect of lncRNA‐HEIH on ESCC tumour growth. The asterisk indicates a significant change (P < .05). The data are the mean ± standard deviations