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. 2020 Aug 4;24(18):10768–10784. doi: 10.1111/jcmm.15702

Figure 4.

Figure 4

BMP2 signalling enhanced migration and invasion of NSCLC cells. A‐C, 1 × 104 LLCs, NCI‐H1373 cells and A549 cells in culture media without FBS were placed on the upper layer of cell culture insert with polycarbonate membrane (Transwell@, 8.0 μm pore size, Corning). The complete culture media with or without 20 ng/mL BMP2 were placed below the cell permeable membrane. The cells migrating to the bottom of the membrane were stained with crystal violet and counted. Representative photographs were shown. Scale bars, 100 μM. Average cell numbers of at least three fields were shown on the right. The P value was based on Student's t test. (*P < .05, **P < .01). D‐E, Corning cell culture insert with polycarbonate membrane (Transwell@, 8.0μm pore size, Corning) were pre‐treated with 10:1 DMEM and matrigels (BD BioSciences). 1 × 105 LLCs and A549 cells were treated the same way as LLCs in (A). The cells invading to the bottom were stained with crystal violet and counted. Representative photos were shown. Scale bars, 100 μM. Average cell numbers of at least three fields were shown on the right. The P value was based on Student's t test. (*P < .05, **P < .01)