FIGURE 8.

SYNCRIP is involved in lncNT5E‐mediated proliferation and EMT of PC cells. A‐C, In CFPAC cells transfected with SYNCRIP siRNA, the SYNCRIP expression level down‐regulated by si‐SYNCRIP was confirmed by Western blotting (C), the proliferation activity was determined by CCK‐8 assay (A), the migration and invasion capacity was detected by Transwell assays (B), and changes in the E‐cad, N‐cad and vimentin protein levels were verified by Western blotting (C). D‐F, Inhibition of proliferation, migration, invasion and changes in EMT‐related markers in lncNT5E‐depleted PC cells was reversed by SYNCRIP overexpression. D, The protein levels of SYNCRIP were up‐regulated, and EMT‐related marker expression was rescued after transfection with the SYNCRIP overexpression plasmid into CFPAC cells with lncNT5E depletion, as detected by Western blotting. E and F, Restoration of SYNCRIP expression significantly ameliorated the lncNT5E depletion‐induced suppression of CFPAC cell proliferation (E), invasion and migration (F). The results are shown as the protein intensity relative to that of GAPDH. Values are presented as the mean ± SD. *P < .05, **P < .01, ***P < .001