Fig. 1.

Comparison of plasmid- and RCA-based workflows for viral rescue. The plasmid-based system involves the transformation of a plasmid into bacteria. The bacteria are then selected and propagated using antibiotic enriched media, and the plasmid is purified from the bacteria and transfected into the cell type of interest. The red exclamation points indicate points during the workflow where mutations and error can be introduced or enhanced. The RCA-based system involves amplification of the plasmid using random hexamers to produce the hyperbranched product. This product can then be directly transfected into cells.