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. 2020 Sep 4;46(5):1755–1764. doi: 10.3892/ijmm.2020.4720

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Association between CYLD and miR-181b in SW480 cells. (A) Results of RT-qPCR showing CYLD mRNA expression in SW480 cells. (B) Results of western blot analysis showing the protein expression levels of CYLD in SW480 cells. (C) MiR-181b directly targets CYLD 3'UTR. (D) Results of dual luciferase assay in cells co-transfected with a miR-181b recombinant vector and the CYLD target gene. ^*P<0.05, compared with the BC group; ^#P<0.05, compared with the miR-181b group; ^*P<0.05, compared with the miR-NC group. BC, blank control; NC1, miR-181b overexpression negative control group; NC2, miR-181b silencing negative control group.