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Effects of different doses of FGF23 on ERK phosphorylation and osteoblastic differentiation in rat VSMCs. FGF23 increased the protein expression levels of p-ERK in a (A) dose- and (B) time-dependent manner. FGF23 increased the protein expression levels of (C) Msx2 and (D) Osx2 in a dose-dependent manner. *P<0.05 vs. 0 ng/ml FGF23 treatment. FGF23, fibroblast growth factor 23; ERK, extracellular signal-related kinase; VSMC, vascular smooth muscle cell; p-, phospho-; t-, total-.
