Table 1. Primer sequences used for RT-qPCR.
Targets | Primer sequence |
miR-21 | F: 5’- ACACTCCAGCTGGGTAGCTTATCAGACTGA-3’ |
R: 5’- TGGTGTCGTGGAGTCG-3’ | |
U6 | F: 5’- GCTTCGGCAGCACATATACTAAAAT-3’ |
R: 5’- CAGTGCGTGTCGTGGAGT-3’ | |
Lnc-SNHG12 | F: 5’- GGTGCTCCAGGCAATAACT-3’ |
R: 5’- CTCCCATACAGTCCGAACAT-3’ | |
GAPDH | F: 5’- TCACCAGGGCTGCTTTTAAC-3’ |
R: 5’- GACAAGCTTCCCGTTCTCAG-3’ |
Notes: MiR-21, MicroRNA-21; Lnc-SNHG12, Long-non coding RNA-SNHG12; ZNF217; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.