Table 1. List of primers of the antioxidant enzymes in qRT-PCR analysis.
Gene symbol | Accession number | Primer sequence (5'–3') | Product size | Tm(°C) |
CuZn- SOD | NM205064 | F: CGCAGGTGCTCACTTTAATCC | 119bp | 57 |
R: CTATTTCTACTTCTGCCACTCCTCC | ||||
Mn-SOD | NM204211 | F: CACTCTTCCTGACCTGCCTTACG | 146bp | 57 |
R: TTGCCAGCGCCTCTTTGTATT | ||||
CAT | NM001031215 | F: CTGTTGCTGGAGAATCTGGGTC | 160bp | 61 |
R: TGGCTATGGATGAAGGATGGAA | ||||
GSH-Px | NM001277853 | F: TTGTAAACATCAGGGGCAAA | 140bp | 61 |
R: TGGGCCAAGATCTTTCTGTAA |