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. 2020 Jul 4;12(17):16867–16886. doi: 10.18632/aging.103585

Table 2. List of primers of the apoptotic genes in qRT-PCR analysis.

Gene symbol Accession number Primer sequence (5′–3′) Product size Tm (°C)
caspase-3 NM_009810 F: ACATGGGAGCAAGTCAGTGG 149bp 60
R: CGTCCACATCCGTACCAGAG
caspase-9 NM_015733 F: GAGGTGAAGAACGACCTGAC 103bp 57
R: AGAGGATGACCACCACAAAG
Bax NM_007527 F: ATGCGTCCACCAAGAAGC 163bp 61
R: CAGTTGAAGTTGCCATCAGC
Bak NM_007523 F: CGCTACGACACAGAGTTCCA 175bp 60
R: CACGCTGGTAGACGTACAGG
Bcl-2 NM_009741 F: AGCCTGAGAGCAACCCAAT 159bp 59
R: AGCGACGAGAGAAGTCATCC
Bcl-xL NM_009743 F: TGTGGATCTCTACGGGAACA 117bp 59
R: AAGAGTGAGCCCAGCAGAAC