Table 2. List of primers of the apoptotic genes in qRT-PCR analysis.
Gene symbol | Accession number | Primer sequence (5′–3′) | Product size | Tm (°C) |
caspase-3 | NM_009810 | F: ACATGGGAGCAAGTCAGTGG | 149bp | 60 |
R: CGTCCACATCCGTACCAGAG | ||||
caspase-9 | NM_015733 | F: GAGGTGAAGAACGACCTGAC | 103bp | 57 |
R: AGAGGATGACCACCACAAAG | ||||
Bax | NM_007527 | F: ATGCGTCCACCAAGAAGC | 163bp | 61 |
R: CAGTTGAAGTTGCCATCAGC | ||||
Bak | NM_007523 | F: CGCTACGACACAGAGTTCCA | 175bp | 60 |
R: CACGCTGGTAGACGTACAGG | ||||
Bcl-2 | NM_009741 | F: AGCCTGAGAGCAACCCAAT | 159bp | 59 |
R: AGCGACGAGAGAAGTCATCC | ||||
Bcl-xL | NM_009743 | F: TGTGGATCTCTACGGGAACA | 117bp | 59 |
R: AAGAGTGAGCCCAGCAGAAC |