Table 3. List of primers of the inflammatory mediators in qRT-PCR analysis.

Gene symbol	Accession number	Primer sequence (5′–3′)	Product size	Tm (°C)
NF-κB	NM205134	F: CTGAAACTACTGATTGCTGCTGGA	179bp	62
		R: GCTATGTGAAGAGGCGTTGTGC
IκB	NM204588	F:TGAGGACGAGGACGATAAGC	146bp	58.8
		R: ACAACGTGATCGCCATTACCTG
COX-2	NM001167718	F: CTTAAATTGAGACTTCGCAAGGATG	165bp	62
		R: TGGGACCAAGCCAAACACCT
IL-1β	Y15006	F: CAGCCTCAGCGAAGAGACCTT	106bp	60
		R: CACTGTGGTGTGCTCAGAATCC
IL-8	HM179639	F: CTGGCCCTCCTCCTGGTT	105bp	60
		R: GCAGCTCATTCCCCATCTTTAC
IL-6	NM001314054.1	F:ACAAAGCCAGAGTCCTTCAGAG	86bp	60
		R:GCCACTCCTTCTGTGACTCC
TNF-α	NM204267	F: CCCCTACCCTGTCCCACAA	100bp	58
		R: TGAGTACTGCGGAGGGTTCAT
IL-2	NM_001303244.1	F: TGTGGAATGGCGTCTCTGTC	125bp	60
		R: AGTTCAATGGGCAGGGTCTC
IL-4	NM_021283.2	F: ATGGATGTGCCAAACGTCCT	78bp	60
		R: AAGCACCTTGGAAGCCCTAC
IL-10	NM_010548.2	F: TGCCTGCTCTTACTGACTGG	79bp	60
		R: CTGGGAAGTGGGTGCAGTTAT
β-actin	NM_007393	F:GCTGTGCTATGTTGCTCTAG	117bp	60.9
		R:CGCTCGTTGCCAATAGTG