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. 2020 Aug 5;12(17):16820–16836. doi: 10.18632/aging.103479

Figure 2.

Figure 2

Inhibition of NET formation alleviated neutrophilic airway inflammation in NA model, rather than degrading the NETs DNA. (A) Flow chart for sivelestat or DNase I administration intraperitoneally. (B) HE staining results showed that sivelestat could alleviate inflammation while DNase I could not (200X). (C–E) Total cells, differential count of cells, and total protein were measured after treatment with sivelestat or DNase I in the BALF of NA mice. (F) Airway resistance was measured after methacholine treatment. (G) Concentration of CXCL1, CXCL2 and CXCL8 in the BALF supernatant of NA mice was measured by ELISA. (H) CXCL1, CXCL2, and CXCL8 expression was measured by Immunohistochemistry (200X). #: P<0.05, ##: P<0.01, ###: P<0.001, ####: P<0.0001 vs control group. *: P<0.05, **: P<0.01, ***: P<0.001, ****: P<0.0001 vs NA group. ns = no statistical difference vs NA group.