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. 2020 Sep 11;12(17):17099–17113. doi: 10.18632/aging.103641

Figure 6.

Figure 6

Deacetylation activity of SIRT6 on H3K9ac, H3K18ac and H3K56c is not required for spermatogenesis. (A) The levels of H3K9ac in Sirt6+/+ and Sirt6-/- testes were detected by western blot. 8-week mice, n=3. (B) Localization of H3K9ac in Sirt6+/+ and Sirt6-/- seminiferous tubules. Testes sections of Sirt6+/+ and Sirt6-/- stained with SYCP3 (green) and H3K9ac (red) antibodies. 8-week mice, n=4. (C) Quantification of H3K9ac intensity of round spermatids and elongated spermatids in the Sirt6+/+ and Sirt6-/- mice. Sirt6+/+, 15.82±0.48; Sirt6-/-, 14.78±0.58. 8-week mice, n=4; 200 cells were used for each group. Data are presented as mean ± SEM. (D) The levels of H3K18ac in Sirt6+/+ and Sirt6-/- testes were detected by western blot. 8-week mice, n=3. (E) The levels of H3K56ac in Sirt6+/+ and Sirt6-/- testes were detected by western blot. 8-week mice, n=3. (F) A functional diagram of Sirt6 in spermatogenesis.