Figure 5.
Slug is a target of miR-506-3p. (A) Bioinformatics analysis prediction using the online website TargetScan determined the putative binding site of Slug and miR-506-3p. (B and C) Luciferase activity of (B) Huh7 and (C) Hep3B cells co-transfected with miR-506-3p or miR-NC and Slug-Wt or Slug-Mut. (D) Correlation between Slug and miR-506-3p (r=−0.5849, P<0.001). (E and F) Slug protein expression in (E) Huh7 and (F) Hep3B cells transfected with miR-506-3p, miR-506-3p + HOXA11-AS, miR-506-3p + Vecter or miR-NC determined by western blot analysis. GAPDH was used as an internal reference. *P<0.05 vs. respective control.