Figure 6.

Slug abrogates the inhibitory effect on HCC progression induced by HOXA11-AS silencing. Huh7 and Hep3B cells were transfected with si-HOXA11-AS + Slug, si-HOXA11-AS, si-HOXA11-AS + control, or si-NC. (A and B) Slug protein expression in (A) Huh7 and (B) Hep3B cells at 24 h post-transfection. GAPDH was used as an internal reference. The (C) proliferative and (D) invasive ability of transfected Huh7 and Hep3B cells were measured by CCK-8 and Transwell assays, respectively. (E-H) mRNA and protein expression of the EMT markers E-cadherin, N-cadherin and vimentin in transfected Huh7 (E and G) and Hep3B (F and H) cells was examined by RT-qPCR and western blot analysis. GAPDH was used as an internal reference. ^*P<0.05.