Figure 5.

fmhA mediates serine insertion in a femAB depleted background. A, the peptidoglycan of femAB pHTT4, femAB fmhA pHTT4, or femAB fmhC pHTT4 was isolated and treated with mutanolysin, ϕ11 hydrolase, and cyanogen bromide (CnBr. B, MALDI-TOF spectra of purified H₆-CWS anchor peptides. C, the major m/z values are listed; see Table 3 for detailed m/z. D, diagrams of the assigned structures for peak i and peak iv. Of note, S₁G₄ in structure iv depicts the ratio between glycyl and seryl residues; the linkage unit (N to C terminus) could be GGSGG or SGGGG.